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KMID : 0385020020180030110
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Korean Journal of Laboratory Animal Science
2002 Volume.18 No. 3 p.110 ~ p.113
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Hexokinase Activity and Subsequent Development of Fresh Bovine Embryos from In Vitro Fertilization
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Kim, Kil Soo
Choe, Nonghoon/Lee, Ho-Joon/Ryoo, Zae Young
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Abstract
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To investigate the Embden-Meyahof pathway (EMP) in bovine embryos, individual 1-cell to blastocyst stage embryos produced from in vitro maturation (IVM) and in vitro fertilization (IVF) systems were subjected to measure hexokinase activity using a cyclinic enzyme method. In the present study, we founded that Ca^++, Mg^++ free phosphate buffered saline, PBS (-), could be used as a reaction solution instead of Tris-HCL buffer and that after measurement the viability of the embryo could be maintained followd by subsequent development. In embryos at all stages examined, the level of hexokinase activity was increased by adding the substrate. When subsequent development after measurement was examined using the 4-cell stage embryos, the development rate to the blastocyst state was not significantly correlated to the hexokinase activity. The results suggest that the onset of glucose metabolism in the early embryos may be directly related to the synthesis of none or more key glycolytic enzyme.
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